CD45RB Antibody
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P08575
Host
Rabbit
Reactivity
Human
Immunogen
Human CD45RB protein was used as the immunogen for the CD45RB antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD45RB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD45RB antibody is available for research use only.
Storage Conditions
Store the CD45RB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD45RB antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Western blot testing of human spleen lysate with CD45RB antibody. Expected molecular weight: 147-220 kDa depending on glycosylation level.
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