ZFYVE28 Antibody

ZFYVE28 belongs to the lst-2 family. It contains 1 FYVE-type zinc finger. The FYVE-type zinc finger mediates the interaction with phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes when not mono-ubiquitinated at Lys-87. Mono-ubiquitination at Lys-87 prevents binding to phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes. ZFYVE28 is a negative regulator of epidermal growth factor receptor (EGFR) signaling. It acts by promoting EGFR degradation in endosomes when not mono-ubiquitinated. The FYVE domain has been identified in a number of unrelated signaling molecules. This protein functions to recruit SMAD2 to the transforming growth factor-beta receptor. The FYVE domain is required to maintain the normal localization of this protein but is not involved in mediating interaction with SMADs.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL

UniProt

Q9HCC9

Host

Mouse

Reactivity

Human

Immunogen

A recombinant full-length human protein was used as the immunogen for this ZFYVE28 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

LST2/2426

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the ZFYVE28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ZFYVE28 antibody is available for research use only.

Storage Conditions

Store the ZFYVE28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the ZFYVE28 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human gastric carcinoma with ZFYVE28 antibody (clone LST2/2426) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.