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PSAP Antibody / Prostate Specific Acid Phosphatase

Recognizes a protein of 52kDa, identified as prostate specific acid phosphatase (PSAP) . This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P15309

Host

Mouse

Reactivity

Human

Immunogen

Prostatic acid phosphatase purified from human seminal plasma was used as the immunogen for this PSAP antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PASE/4LJ

Applications

IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the PSAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PSAP antibody is available for research use only.

Storage Conditions

Store the PSAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the PSAP antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human prostate carcinoma with PSAP antibody (clone PASE/4LJ) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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