Calcineurin B Antibody / PPP3R1
Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P63098
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length human Calcineurin B protein was used as the immunogen for the Calcineurin B antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
BE2.1
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Calcineurin B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Calcineurin B antibody is available for research use only.
Storage Conditions
Store the Calcineurin B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Calcineurin B antibody should be determined by the researcher.
Location
Cytoplasmic
Frequently Asked Questions
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