Granzyme B Antibody
Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P10144
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 73-187) was used as the immunogen for the Granzyme B antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
GZMB/3014
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Granzyme B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Granzyme B antibody is available for research use only.
Storage Conditions
Store the Granzyme B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Granzyme B antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human spleen lysate with Granzyme B antibody (clone GZMB/3014) . Predicted molecular weight: 29-37 kDa.
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