EGLN1 Antibody / PHD2
366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q9GZT9
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 1-24 of human PHD2/EGLN1 were used as the immunogen for the EGLN1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
366G/76/3
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the EGLN1 / PHD2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EGLN1 / PHD2 antibody is available for research use only.
Storage Conditions
Store the EGLN1 / PHD2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the EGLN1 / PHD2 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
SDS-PAGE analysis of purified, BSA-free EGLN1 antibody (clone 366G/76/3) as confirmation of integrity and purity.
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