BOB.1 Antibody

BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q16633

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB.1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

BOB1/2422

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the BOB.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This BOB.1 antibody is available for research use only.

Storage Conditions

Store the BOB.1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the BOB.1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human tonsil with BOB.1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.