Cytokeratin 20 Antibody / CK20
His MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20) . KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P35900
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 196-323) was used as the immunogen for this Cytokeratin 20 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SPM140
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Cytokeratin 20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Cytokeratin 20 antibody is available for research use only.
Storage Conditions
Store the Cytokeratin 20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Cytokeratin 20 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human HT29 cell lysate with Cytokeratin 20 antibody (clone SPM140) . Predicted molecular weight ~46 kDa.
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