MSH6 Antibody
The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P52701
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 374-540) was used as the immunogen for the MSH6 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
MSH6/3091
Applications
ELISA, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MSH6 antibody is available for research use only.
Storage Conditions
Store the MSH6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the MSH6 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MSH6/3091) and Phalloidin (red) .
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