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UPK1A Antibody / Uroplakin 1A

Component of the asymmetric unit membrane (AUM) ; a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O00322

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 114-173 were used as the immunogen for the UPK1A antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TTSP21-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the UPK1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This UPK1A antibody is available for research use only.

Storage Conditions

Store the UPK1A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the UPK1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human urothelial carcinoma with UPK1A antibody (clone TTSP21-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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