PCNA Antibody

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P12004

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A rat PCNA/protein A fusion protein was used at the immunogen for this PCNA antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PC5

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PCNA antibody is available for research use only.

Storage Conditions

Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the PCNA antibody should be determined by the researcher.

Location

Predominantly nuclear, some cytoplasmic

Image Legend

IHC staining of FFPE human tonsil with PCNA antibody (clone PC5) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.