PAX5 Antibody

The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding domain, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis; therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q02548

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 235-382) was used as the immunogen for the PAX5 antibody.

Clonality

Monoclonal

Isotype

IgG1 λ

Clone

PAX5/3735

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the PAX5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PAX5 antibody is available for research use only.

Storage Conditions

Store the PAX5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the PAX5 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of human tonsil with PAX5 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.