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Recombinant YB-1 Antibody

Y-box binding protein-1 (YBX1) is the prototypic member of the cold shock protein family that fulfills numerous cellular functions. In the nucleus, YBX1 protein orchestrates transcription of proliferation-related genes, whereas in the cytoplasm it associates with mRNA and directs translation. In human tumor entities, such as breast, lung and prostate cancer, cellular YBX1 expression indicates poor clinical outcome, suggesting that YBX1 is an attractive marker to predict patients' prognosis.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P67809

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human full-length YBX1 protein was used as the immunogen for this recombinant YB-1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RYBX1/2430

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant YB-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant YB-1 antibody is available for research use only.

Storage Conditions

Store the recombinant YB-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant YB-1 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of FFPE human prostate carcinoma with recombinant YB-1 antibody (clone rYBX1/2430) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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