Recombinant NGF Receptor Antibody / p75NTR / CD271

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR) . NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL (not suitable for frozen sample testing), Western blot: 1-2 µg/mL

UniProt

P08138

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 281-421) was used as the immunogen for this recombinant NGF Receptor antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RNGFR/1965

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant NGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant NGF Receptor antibody is available for research use only.

Storage Conditions

Store the recombinant NGF Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant NGF Receptor antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

Western blot testing of human U-87 cell lysate with recombinant NGF Receptor antibody (clone rNGFR/1965) . Predicted molecular weight: 45-75 kDa depending on glycosylation level.