NCAM Antibody / CD56

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas) . It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma) . Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL

UniProt

P13591

Host

Mouse

Reactivity

Human, Rat

Immunogen

A recombinant human protein was used as the immunogen for this NCAM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ERIC-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the NCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This NCAM antibody is available for research use only.

Storage Conditions

Store the NCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the NCAM antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human colon with NCAM antibody (clone ERIC-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.