MSH2 Antibody

Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) . Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL

UniProt

P43246

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 327-427) was used as the immunogen for this MSH2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MSH2/2622

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the MSH2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MSH2 antibody is available for research use only.

Storage Conditions

Store the MSH2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the MSH2 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human colon carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.