MDM2 Antibody

MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53. It can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Overexpression of MDM2 protein is detected in a variety of cancers.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q00987

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 154-167 were used as the immunogen for the MDM2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SMP14

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the MDM2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MDM2 antibody is available for research use only.

Storage Conditions

Store the MDM2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the MDM2 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human HepG2 cell lysate with MDM2 antibody (clone SMP14) . Predicted molecular weight: ~55 kDa but can be observed at up to ~90 kDa.