MDM2 Antibody
MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53. It can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. Overexpression of MDM2 protein is detected in a variety of cancers.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q00987
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 154-167 were used as the immunogen for the MDM2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SMP14
Applications
ELISA, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the MDM2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MDM2 antibody is available for research use only.
Storage Conditions
Store the MDM2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the MDM2 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Frequently Asked Questions
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