Recombinant Erythropoietin Antibody / EPO

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL

UniProt

P01588

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids 28-162 were used as the immunogen for the recombinant Erythropoietin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

ERTP-1

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Erythropoietin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Erythropoietin antibody is available for research use only.

Storage Conditions

Store the recombinant Erythropoietin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Erythropoietin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human kidney with recombinant Erythropoietin antibody (clone ERTP-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.