EpCAM Antibody
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-2ug/million cells
UniProt
P16422
Host
Mouse
Reactivity
Human
Immunogen
Human colon cancer HT-29 cells were used as the immunogen for the EpCAM antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
HEA125
Applications
IF, IHC-P, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EpCAM antibody is available for research use only.
Storage Conditions
Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the EpCAM antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone HEA125, green) and Reddot nuclear stain (red) .
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