Desmoglein 3 Antibody / DSG3

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3) . This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC) . Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P32926

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 379-491) was used as the immunogen for the Desmoglein 3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

DSG3/2839

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Desmoglein 3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Desmoglein 3 antibody is available for research use only.

Storage Conditions

Store the Desmoglein 3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Desmoglein 3 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human esophageal carcinoma with Desmoglein 3 antibody (clone DSG3/2839) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.