EpCAM Antibody
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-3 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P16422
Host
Mouse
Reactivity
Human, Dog, Cat
Immunogen
A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
EGP40/1373
Applications
WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EpCAM antibody is available for research use only.
Storage Conditions
Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the EpCAM antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EGP40/1373, green) and Reddot nuclear stain (red) .
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