LSP1 Antibody / Lymphocyte Specific Protein 1

LSP1 is a hematopoietic-expressed gene that encodes an F-actin-binding, leukocyte-specific (including B and T lymphocytes, granulocytes and macrophages), phosphoprotein. However, mRNA splice variants that do not encode the lympho-specific protein are expressed from this gene in nonlymphoid cell lines as well (myocytes, stromal cells and fibroblasts), suggesting pp52 has a divergent role in signal transduction. The pp52 (LSP1) locus maps to human chromosome 11p15.5, which is implicated in tumor-related chromosomal translocations found in chronic lymphocytic leukemia. The pp52 promoter contains key elements that control transcriptional activity including an initiator specifying the unique 5' terminus of pp52 mRNA, tandem pairs of Ets and SP1 motifs, and a single C/EBP motif. LSP1 binds the cytoskeleton and has been implicated in affecting cytoskeletal remodeling in a variety of leukocyte functions, including cell motility and chemotaxis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P33241

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 174-278) was used as the immunogen for this LSP1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LSP1/3025

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the LSP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This LSP1 antibody is available for research use only.

Storage Conditions

Store the LSP1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the LSP1 antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

IHC staining of FFPE human lymphoma with LSP1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.