CK1 Antibody / Cytokeratin 1 / Keratin 1

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8) . Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10) . Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL

UniProt

P04264

Host

Mouse

Reactivity

Human, Rat

Immunogen

A recombinant full-length human Cytokeratin 1 protein was used as the immunogen for this CK1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

KRT1/1840

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CK1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CK1 antibody is available for research use only.

Storage Conditions

Store the CK1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CK1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human cervical carcinoma with CK1 antibody (clone KRT1/1840) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.