Cytokeratin 1 Antibody / CK1 / Keratin 1

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8) . Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10) . Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL

UniProt

P04264

Host

Mouse

Reactivity

Human, Rat

Immunogen

Amino acids VRFVSTSYSGVTR from the C-terminal portion of the protein was used as the immunogen for this Cytokeratin 1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

LHK1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Cytokeratin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytokeratin 1 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytokeratin 1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human cervix with Cytokeratin 1 antibody (clone LHK1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.