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ARF1 Antibody / ADP-ribosylation factor 1

The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Product Specifications

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P84077

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant full-length human ARF1 protein was used as the immunogen for this ARF1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

ARF1/2117

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the ARF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ARF1 antibody is available for research use only.

Storage Conditions

Store the ARF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Product Datasheet

https://www.nsjbio.com/tds-pdf/arf1-antibody-adp-ribosylation-factor-1-arf12117-v7901

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the ARF1 antibody should be determined by the researcher.

CAS Number

9007-83-4

Location

Cytoplasmic (Golgi)

Image Legend

IHC staining of FFPE human brain with ARF1 antibody (clone ARF1/2117) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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