ARF1 Antibody / ADP-ribosylation factor 1

The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P84077

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant full-length human ARF1 protein was used as the immunogen for this ARF1 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

ARF1/2117

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the ARF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ARF1 antibody is available for research use only.

Storage Conditions

Store the ARF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the ARF1 antibody should be determined by the researcher.

Location

Cytoplasmic (Golgi)

Image Legend

IHC staining of FFPE human brain with ARF1 antibody (clone ARF1/2117) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.