ARF1 Antibody / ADP-ribosylation factor 1
The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P84077
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids SNQLRNQ were used as the immunogen for this ARF1 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
3F1
Applications
IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the ARF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ARF1 antibody is available for research use only.
Storage Conditions
Store the ARF1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the ARF1 antibody should be determined by the researcher.
Location
Cytoplasmic (Golgi)
Image Legend
SDS-PAGE analysis of purified, BSA-free ARF1 antibody (clone 3F1) as confirmation of integrity and purity.
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