Recombinant TIMP2 Antibody

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2) . It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B) . TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P16035

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids DSGNDIYGNPI (N-terminal region) were used as the immunogen for the recombinant TIMP2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RTIMP2/2335

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant TIMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant TIMP2 antibody is available for research use only.

Storage Conditions

Store the recombinant TIMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant TIMP2 antibody should be determined by the researcher.

Location

Cytoplasmic, cell surface, secreted

Image Legend

IHC staining of FFPE human placenta with recombinant TIMP2 antibody (clone rTIMP2/2335) . Required HIER: requires boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.