ITGB3 Antibody / Integrin beta 3 / CD61

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain) . It associates with the IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61) .The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P05106

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 385-490) was used as the immunogen for this ITGB3 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ITGB3/2597

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the ITGB3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ITGB3 antibody is available for research use only.

Storage Conditions

Store the ITGB3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the ITGB3 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human spleen with ITGB3 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.