Recombinant RPA32 Antibody / Replication Protein A2
Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) . RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively) . It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
Product Specifications
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL
UniProt
P15927
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human full-length Replication protein A2 protein was used as the immunogen for this recombinant RPA32 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
RPA2/3140R
Type
Recombinant
Applications
WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant RPA32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant RPA32 antibody is available for research use only.
Storage Conditions
Store the recombinant RPA32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant RPA32 antibody should be determined by the researcher.
Image Legend
Western blot testing of human MCF7 and T47D cell lysate with recombinant RPA32 antibody. Expected molecular weight ~32 kDa.
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