EGFR Antibody / EGF Receptor

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P00533

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human Epidermal growth factor receptor protein was used as the immunogen for the EGFR antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

RGFR/1667

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This EGFR antibody is available for research use only.

Storage Conditions

Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.

Image Legend

Western blot testing of human A431 cell lysate with EGFR antibody (clone rGFR/1667) . Expected molecular weight: ~134/170 kDa (unmodified/glycosylated) .