Recombinant IgM Heavy Chain Antibody
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P01871
Host
Mouse
Reactivity
Human
Immunogen
Heavy chain of human IgM was used as the immunogen for the recombinant IgM Heavy Chain antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RIGHM/2558
Type
Recombinant
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant IgM Heavy Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant IgM Heavy Chain antibody is available for research use only.
Storage Conditions
Store the recombinant IgM Heavy Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Product Datasheet
https://www.nsjbio.com/tds-pdf/recombinant-igm-heavy-chain-antibody-righm2558-v7868
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant IgM Heavy Chain antibody should be determined by the researcher.
Image Legend
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Lentiviral human CCDC34 shRNA (UAS) - Lentiviral human CCDC34 shRNA (UAS, GFP) (100)
Lentiviral human HEMGN shRNA (UAS) - Lentiviral human HEMGN shRNA (UAS) (25)