Recombinant SOX2 Antibody

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P48431

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 176-305) was used as the immunogen for the recombinant SOX2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

SOX2/3811R

Type

Recombinant

Applications

ELISA, WB, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant SOX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant SOX2 antibody is available for research use only.

Storage Conditions

Store the recombinant SOX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant SOX2 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SOX2/3811R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.