CD50 Antibody / ICAM-3
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI) . CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C.
Product Specifications
CAS Number
9007-83-4
Specifications
Blocks binding of CD11a/LFA-1 to CD50/ICAM-3 (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL
UniProt
P32942
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the CD50 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CG106
Applications
Blocking, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD50 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD50 antibody is available for research use only.
Storage Conditions
Store the CD50 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD50 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
SDS-PAGE analysis of purified, BSA-free CD50 antibody (clone CG106) as confirmation of integrity and purity.
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