FOXA1 Antibody

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1, and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER) -positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P55317

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 372-472) was used as the immunogen for the FOXA1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FOXA1/1516

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the FOXA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This FOXA1 antibody is available for research use only.

Storage Conditions

Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the FOXA1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human PIN (Prostatic Intraepithelial Neopplasia) with FOXA1 antibody (clone FOXA1/1516) . HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.