Granzyme B Antibody

Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P10144

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 73-187) was used as the immunogen for the Granzyme B antibody.

Clonality

Monoclonal

Isotype

IgG

Clone

GZMB/3056

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Granzyme B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Granzyme B antibody is available for research use only.

Storage Conditions

Store the Granzyme B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Granzyme B antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human spleen with Granzyme B antibody (clone GZMB/3056) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.