MSH6 Antibody

The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P52701

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 374-540) was used as the immunogen for the MSH6 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MSH6/2927

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MSH6 antibody is available for research use only.

Storage Conditions

Store the MSH6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the MSH6 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human lymph node with MSH6 antibody (clone MSH6/2927) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.