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CLEC9A Antibody / DNGR-1

This MAb recognizes a protein of 27kDa, identified as CLEC9A (C-type lectin domain family 9 member A) . Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL

UniProt

Q8RU4

Host

Mouse

Reactivity

Human

Immunogen

RBL-2H3 cells expressing human CLEC9A fused to an HA epitope. Its epitope maps to within amino acids 50-110.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

8F9

Applications

FACS, IF

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the CLEC9A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CLEC9A antibody is available for research use only.

Storage Conditions

Store the CLEC9A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the CLEC9A antibody should be determined by the researcher.

Image Legend

SDS-PAGE analysis of purified, BSA-free CLEC9A antibody (clone 8F9) as confirmation of integrity and purity.

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