Glucose 6-Phosphate Isomerase Antibody / GPI

In addition to it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P06744

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human full-length protein was used as the immunogen for the Glucose 6-Phosphate Isomerase antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

CPTC-GPI-1

Applications

WB, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Glucose 6-Phosphate Isomerase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Glucose 6-Phosphate Isomerase antibody is available for research use only.

Storage Conditions

Store the Glucose 6-Phosphate Isomerase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Glucose 6-Phosphate Isomerase antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human breast carcinoma with Glucose 6-Phosphate Isomerase antibody (clone CPTC-GPI-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.