Glycoprotein 2 Antibody / GP2 / ZAP75

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD) . GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry: 1-2 µg/mL

UniProt

P55259

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 35-179) was used as the immunogen for this Glycoprotein 2 antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

GP2/3416

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Glycoprotein 2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Glycoprotein 2 antibody is available for research use only.

Storage Conditions

Store the Glycoprotein 2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Glycoprotein 2 antibody should be determined by the researcher.

Location

Cytoplasmic, membranous, secreted

Image Legend

IHC testing of FFPE human pancreas with Glycoprotein 2 antibody (clone GP2/3416) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.