GATA3 Antibody

GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas. Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P23771

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 357-436) was used as the immunogen for the GATA3 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

GATA3/2444

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the GATA3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GATA3 antibody is available for research use only.

Storage Conditions

Store the GATA3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the GATA3 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human breast carcinoma with GATA3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.