NKX2.8 Antibody
The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL
UniProt
O15522
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 10-123) was used as the immunogen for the NKX2.8 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NKX28/2548
Applications
ELISA, FACS, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the NKX2.8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NKX2.8 antibody is available for research use only.
Storage Conditions
Store the NKX2.8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the NKX2.8 antibody should be determined by the researcher.
Image Legend
Western blot testing of human PC-3 and MCF-7 cell lysate with NKX2.8 antibody. Predicted molecular weight ~26 kDa, commonly observed at 26-34 kDa.
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