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NKX2.8 Antibody

The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL

UniProt

O15522

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 10-123) was used as the immunogen for the NKX2.8 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NKX28/2548

Applications

ELISA, FACS, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the NKX2.8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This NKX2.8 antibody is available for research use only.

Storage Conditions

Store the NKX2.8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the NKX2.8 antibody should be determined by the researcher.

Image Legend

Western blot testing of human PC-3 and MCF-7 cell lysate with NKX2.8 antibody. Predicted molecular weight ~26 kDa, commonly observed at 26-34 kDa.

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