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CD15 Antibody / SSEA-1

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P22083

Host

Mouse

Reactivity

Human

Immunogen

Human U937 cells were used as the immunogen for the CD15 antibody.

Clonality

Monoclonal

Isotype

IgM k

Clone

SPM119

Applications

FACS, IF, IHC-P

Purity

PEG precipitation

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD15 antibody is available for research use only.

Storage Conditions

Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD15 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human monocytes with CD15 antibody (clone SPM119) ; Green=isotype control, Red= CD15 antibody.

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