CD15 Antibody / SSEA-1
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P22083
Host
Mouse
Reactivity
Human
Immunogen
Human U937 cells were used as the immunogen for the CD15 antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
SPM119
Applications
FACS, IF, IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD15 antibody is available for research use only.
Storage Conditions
Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD15 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human monocytes with CD15 antibody (clone SPM119) ; Green=isotype control, Red= CD15 antibody.
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