Recombinant FTL Antibody / Ferritin light chain

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III) . Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 0.1-0.2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P02792

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein (amino acids 38-165) was used as the immunogen for the recombinant FTL antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RFTL/1388

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant FTL antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant FTL antibody is available for research use only.

Storage Conditions

Store the recombinant FTL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant FTL antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human pancreas with recombinant FTL antibody (clone rFTL/1388) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.