Fibronectin Antibody
Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P02751
Host
Mouse
Reactivity
Human
Immunogen
FR5 cells, derived by SV40 transformation of human mammary epithelial cells were used as the immunogen for this Fibronectin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
Fn-3
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Fibronectin antibody is available for research use only.
Storage Conditions
Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Fibronectin antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free Fibronectin antibody (clone Fn-3) as confirmation of integrity and purity.
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