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ALDH1A1 Antibody / Acetaldehyde dehydrogenase 1

ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P00352

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial ALDH1A1 protein (AA 315-434) was used as the immunogen for this ALDH1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ALDH1A1/1381

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the ALDH1A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ALDH1A1 antibody is available for research use only.

Storage Conditions

Store the ALDH1A1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the ALDH1A1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human testicular carcinoma with ALDH1A1 antibody (clone ALDH1A1/1381) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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