Recombinant CD21 Antibody
Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Product Specifications
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P20023
Host
Rabbit
Reactivity
Human
Immunogen
A partial protein corresponding to amino acids 142-240 was used as the immunogen for the recombinant CD21 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
CR2/3124R
Type
Recombinant
Applications
FACS, IF, WB, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD21 antibody is available for research use only.
Storage Conditions
Store the recombinant CD21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant CD21 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R, green) and Reddot nuclear stain (red) .
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