Recombinant AKT1 Antibody

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P31749

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 85-189 from the human protein were used as the immunogen for the recombinant AKT1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RAKT1/2491

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant AKT1 antibody is available for research use only.

Storage Conditions

Store the recombinant AKT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.

Location

Nucleus, plasma membrane, cytoplasm

Image Legend

IHC staining of FFPE human prostate carcinoma with recombinant AKT1 antibody (clone rAKT1/2491) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.