HER2 Antibody / ErbB2

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P04626

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human HER2 protein fragment within amino acids 311-462 was used as the immunogen for the HER2 antibody. Its epitope is localized in the extracellular domain.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ERBB2/3078

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This HER2 antibody is available for research use only.

Storage Conditions

Store the HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the HER2 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/3078) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.